Abstract
Binding affinities of several drugs for microfibrous collagen (Avitene) were determined applying dialysis measurement, and binding potential was calculated from the efflux rate of drugs. Bromophenolblue showed the strongest affinity for collagen. Released amount of bromophenolblue for 5 hrs from collagen was about 20% and almost constant during 24 hrs. Comparatively weak binding was observed in salicylic acid, chloramphenicol, epirubicin HC1, and 5-FU. There were considerable time lags in the release profiles of all these drugs when the release profiles were compared with those of the control. Only the release behavior of amikacin sulfate was similar to that of the control. On the other hand, release behaviors of cisplatin and mitomicin C were quite different from those of other drugs. They failed to reach to 100% release even in a prolonged incubation period, suggesting a part of these drugs bound to collagen irreversibly. It was supposed that the affinity of drugs for collagen was dependent on its chemical structure, especially electro-resonance structure, and electro-negative charges.
