Abstract
A MFGM emulsion containing vitamin D3 was administered to rate whose pancreatic duct was ligated or intact. The recovered percentage of vitamin D3 in lymph decreased to 1/70 when pancreatic duct was ligated. Three kinds of MFGM microemulsion, one was with both taurocholate and lipase, one with taurocholate alone, and the other without taurocholate or lipase, were administered to pancreatic duct-ligate rats and the lymphatic absorptions of vitamin D3 were compared. It was found that the absorption is significantly different among them. The microemulsion containing both taurocholate and lipase showed the highest promotion effect of lymphatic absorption of vitamin D3. In gel filtration experiment, we confirmed the difference in composition among three kinds of microemulsion. The properties of MFGM emulsion and tween 80 emulsion were also compared. Lipase was added in MFGM emulsion and tween 80 emulsion and the amout of fatty acid in emulsions was determined. It was found that the lipolysis occurs more easily in MFGM emulsion than in tween 80 emulsion. The ESR analysis of microemulsions, containing taurocholate and lipase, indicated that the membrane fluidity of MFGM microemulsion was higher than that of tween 80 microemulsion. These results suggested that the lipase plays an important role in the promotion effect of lymphatic drug absorption by MFGM, which resulting in the difference of lymphatic absorption of vitamin D3 between MFGM and tween 80 dosage forms.
