Efficient gene transfer using unilamellar fusogenic liposomes

Abstract

We have developed fusogenic liposomes with virus envelope proteins on the surface. These liposomes can introduce their contents (DNA, RNA, and protein) into cell cytoplasm directly by membrane fusion in vitro and in vivo. The liposomes can fuse with cell membrane as efficient as virus particles and can maintain their activity indefinitely in liquid nitrogen. Investigation of new system for future gene therapy is now under way based on these fusogenic liposomes.