1993 Volume 13 Issue 4 Pages 433-442
The purpose of present study was to determine the arrangement of collagen fibrils organizing bone matrix. The materials were diaphyses of long bones (humerus, radias, femurs and tibias), bodies of ribs and parietal bones of 3 year-old mature crab-eating monkeys. After fixation, the fibrous layers of ectoperiosteum were tore from bones. Specimens were treated by 1% trypin solution to digest both osteoblasts and amorphous organic matrix, and examined by high resolution scanning electron microscope.
The results we examined were as follows :
(1) On the surface of diaphyses of long bones, the bodies of ribs and parietal bones, collagen fibrils were organized into bundles. These bundles were 0.7-1μm in diameter.
(2) Many bone canaliculi were opened between collagen fibril bundles. Parts of bundles removed to neighbor bundles around bone canaliculi.
(3) At the part where superficial collagen fibril bundles were comparatively sparse, different arranged bundles were recognized between the superficial bundles.
(4) The surface, which was barely uneven, was dotted with the overlapping area of different arranged bundles.
(5) At the limited uneven area, the arrangement of collagen fibril bundles were irregularly running, and crossing each other. The bone canaliculi between bundles were round in shape.
(6) At the surface of parietal bone, bundles were running from parietal tuberosity to all directions.
