Expression Analysis of mRNAs in Oral Squamous Cell Carcinoma Using Laser Microdissection (Laser Pressure Cell Transfer Type) and Semiquantitative RT-PCR

Abstract

Laser microdissection is a new method for the procurement of targeted cells from tissue section and has enabled the analysis of nucleic acid and protein. However, the contamination of cells, other than target cells and damage of mRNA, has become a problem, since it must adhere the target cells to thermoplastic film after dissection in the conventional laser microdissection. We developed a laser pressure cell transfer method that did not need to use thermoplastic film, and the semiquantitative analysis of the various transcriptional product in oral squamous cell carcinoma and normal mucosal epithelium in the identical sample was carried out by using this method and semiquantitative RT-PCR. As a result of this analysis, the procurement of mRNA from each sample was possible. By accumulating of cases using this analysis, it was indicated to become basic data for the establishment of objective judgment and prognostic evaluation of the cancer's malignancy.