2004 Volume 24 Issue 2 Pages 127-138
Periodontal tissues play a key role in success in tooth transplantation and replantation. We have previously realized the long-term preservation of the activity of periodontal tissues by using the UW solution, a conventional solution for preservation of organs. To establish a longer-term preservation method, we investigated the biological activity of human-periodontal ligament cells stored in solutions containing trehalose. Both the cell adhesion and growth of periodontal ligament cells were better in the UW solution than in physiological saline. Storing controlled-freezing point was better than storing frozen, and cellular functions could be preserved more nicely for a long time by storing controlled-freezing point than by storing frozen. Although the adhesiveness and growth of periodontal ligament cells could not be significantly improved by the addition of trehalose, the usefulness of trehalose was suggested.