Chemi- and Bioluminescent Immunoassay

Abstract

The application of a highly sensitive immunoassay, using chemi- or bioluminescent reaction is reviewed. Their advantages of these reactions are that they can be quantiated rapidly, are relatively stable, possess high specific activity, and can take part in an amplification reactions.
They can be employed in the immunoassay in three different ways.
1. The label can be a chemiluminescent substances such as luminol derivatives.
2. Product from label enzyme can be monitored using chem- or bioluminescent reaction. Examples of these application of a luminescence in the immunoassay are for instance, the use of oxidase enzyme or dehydrogenase enzyme labels that produce hydrogen peroxide or NADH.
Those products are monitored using chemi- or bioluminescent reaction.
3. The label can be a catalyst or cofactor for bioluminescent reaction.