HIGH SELECTIVE ANALYSIS OF A NOVEL NEUROLEPTIC, NE-100, AND ITS METABOLITES UTILIZING ANTIBODY AS A FUNCTIONAL MATERIAL

Abstract

Powerful analytical tools were required for studies on the fate of NE-100, which undergoes extensive metabolism. An analytical method based on immunoaffinity chromatography / radioimmunoassay, using two antibodies with different specificities, was developed for the determination of unchanged NE-100 in human plasma. This tandem-type method was of high sensitivity, sufficient both precision and accuracy, and high specificity for unchanged NE-100. For the exploratory detection of potential metabolites having affinity for sigma receptors, immunoaffinity chromatography combined with liquid chromatography-mass spectrometry (/ mass spectrometry) that can recognize metabolites retaining structural features (i.e., N-dipropyl or N-propyl group) was developed. This hybrid-type method was extremely valuable for the recognition and characterization of such metabolites present in biological fluids at low concentrations. These techniques have proved to be useful for studies on the fate of NE-100.