CERULOPLASMIN CATALYZE OXIDATION OF NITRIC OXIDE

Abstract

We have developed the sensitive and specific detection system for S-nitrosothiols using HPLC coupled with Hg²⁺ and Griess reagent. In this study, we have tested the effect of heavy metal ions and metal proteins for the formation of S-nitrosoglutathione (GSNO) and compared this with the GSNO generation by following oxidation of NO in glutathione (GSH)/ NONOate system. In the presence of Cu ion and ceruloplasmin, GSNO formation was significantly increased. This maximun effect was observed at 0.5μM of Cu and Cp concentration in GSH (10μM)/ NONOate (10μM) system and then, the yield of reaction was 25% approximately (GSNO/ GSH, %) which showed the 25 fold higher formation of GSNO rather than control. In RAW 264 cell culture system, which expressed inducible NO syntase, Cp significantly catalzyed formation of GS-NO rather than Cu ion. These observation indicate that Cp might participate in the formation of GSNO in biological system.