1990 Volume 5 Issue 3 Pages 323-330
A specific radioimmunoassay method was developed for H-Met(O2)-Glu-His-Phe-D-Lys-Phe-NH(CH2)8-NH2(ebiratide)., an analogue of adrenocorticotropic hormone. The sensitivity was 30pg/ml. Recovery of ebiratide from plasma was almost 100%, and the intra and inter-assay coefficients of variation were less than 15%. Plasma levels and urinary excretion of ebiratide were determined in six healthy male volunteers after intravenous administration of ebiratide (150μg). Ebiratide was eliminated from the plasma in a biphasic pattern, with half-lives of 3.2min (first phase) and 3h (second phase), and 0. 76% of the dose was excreted in urine. These results indicate that this radioimmunoassay method is suitable for analyzing the pharmacokinetics of ebiratide.
