Abstract
We isolated fungi colonizing oat (Avena sativa L.) and eucalyptus (Eucalyptus camaldulensis Dehnh) roots to select the strains that promote the seed germination and seedling growth of cabbage. Twenty-nine strains were inoculated into the soil which grows cabbage seedlings. This inoculation test was repeated three times. From the results of the test, we found that MT0011 and EU0013 strains promoted seed germination. In the first inoculation test, seven strains increased the dry weight of cabbage seedling significantly compared with the control. In the second test with the strains that promoted cabbage growth, MT0008 and EU0013 strains significantly increased the dry weight of seedlings. In the third test, only EU0013 significantly increased seedling growth. The ITS region for DNA (ITS 1-5.8SrDNA-ITS2) of 28 isolated strains were sequenced to examine the molecular phylogeny. Isolates were classified into two divisions, Zygomycota and Ascomycota. Nine strains showed the highest similarities with same accession number AF504832 (Uncultured fungus clone D50). MT0011 was clustered with fungi belonging to Drechela sp. from the sequence data on the rDNA ITS region. EU0013 was identified as Penicillium sp. from the morphological feature conidiophore and the sequence of the ITS region.
