Studies of cell activators produced by a microbe isolated from the deep-seawater in Izu-Akazawa, Japan

Abstract

In this study, microbes producing cell activators were isolated from bag lters used for removing detritus from the deep-seawater of Izu-Akazawa, Shizuoka Prefecture, Japan, using normal human dermal broblasts. Consequently, 614 strains were isolated from the lters. Among them, three strains showed a cell-activating eect. The strain No. 265 had the highest eect and was selected for future studies. The strain No. 265 was analyzed for the 16S rRNA fragment sequence analysis. The strain showed the homology matched Vibrio gallaecicus CECT7244T at 98.74％. However, there were some dierences in the biochemical and physiological characteristics between the strain No. 265 and the type strain. The cell activators produced by the strain were extracted by an ammonium sulfate precipitation from the cultivation supernatant and isolated by molecular weight fractionation. As a result, over 3 kDa of molecular weight fractionations showed a cell-activating eect. Furthermore, in examining homology of protein between over 3 kDa fractionation and human basic broblast growth factor (human bFGF) as the main cell growth factor using enzyme-linked immunosorbent assay (ELISA), it was found that this fractionation showed no antigen-antibody reaction with human bFGF. As a result, it was suggested that the strain No. 265 produced cell activators dierent from human bFGF.