2022 Volume 23 Issue 2 Pages 65-73
Regarding the characteristics of deep seawater (DSW), one of the resources that matches the concept of sustainable development goals (SDGs), is that it contains various kinds of useful substances for human life unlike solar, wave, wind and geothermal resources. In this study, microorganisms from DSW which have not been fully utilized were the focus. Especially, lactic acid bacteria which have been used in the health care industry were chosen among them. Four isolates were randomly selected from 19 lactic acid bacteria candidates isolated from DSW at Izu-Akazawa and were identified species by 16S rDNA sequence analysis. As a result, all of four isolates were identified as Lactiplantibacillus plantarum. There was a slight difference in physiological and biochemical characteristics between the isolates from DSW and the type strain of L. plantarum. Furthermore, there was also a little difference in enzyme production between the isolates and the type strain. Then, it was suggested that the culture supernatant of the isolate BF1‒13 increased expression of tight junction related proteins (TJs) and aquaporin 3 (AQP3) genes in vitro using normal Madin-Darby canine kidney (MDCK) cells. On the other hand, there was no effect in the supernatant of the type strain.
https://ror.org/048nxq511 