2000 Volume 1 Issue 1 Pages 13-18
Deep sea water is recently under trial as an efficacious reagent for a cure of atopic dermatitis. However, the biologically effective substances, including cytotoxic compounds, in deep sea water are almost unknown. We investigated the effects of deep sea water on function of mouse macrophage in vitro. We found that 1) mitochondrial activity, active oxygen and NO production in macrophage cells cultured with stimulants were enhanced by pretreatment with deep sea water in depth dependent manner; 2) on the other hand, treatment with deep sea water reduced NO production of preactivated cells in depth dependent manner; 3) the both activities of deep sea water stored in 4°C for one year were lower than that stored in -30°C. These results indicate that substances which are able to affect the immune response exist in deep sea water. The immune response is different way and dependent on host condition.
