Studies on the Protein Fractionation with New Salting-out Method and its Some Applications

Abstract

This report presents studying and applying attempted of new method of fractionation of protein with salting-out by ammonium sulfate, turbidimetric titration, previously reported by other authors. That principle is using of ΔT/ΔC, in which ΔT is increment of degree of turbidity and ΔC is increment of degree of saturation of ammonium sulfate. It was convinced that this method is very convenient and suitable on the fractionation of ovalbumin, egg white and normal human serum, so this method is applied to fractionation of protein extracted of fish muscle, clam muscle, clam hepatopancreas crystalline style and human plasma. The good results were obtained from the application of this method, and each extracts are fractionated vividly. The results were shown from figure 1 to figure 10.