Abstract
By the improving of the condition at the time of sterilization, the fall of standard curve of cystine was removed, but the recovery was still undesirable.
Therefore, the author re-examined the nutritional requirement by the test organism, and invented a minimal medium.
The recovery by this minimal medium showed the satisfactory results on the analysis of cystine in serum, urine and foods.
The character of this minimal medium was partial modification of uniform medium which was established by Gakuzo Tamura et al.