Preparation of a Glucose-Sensing Electrode Based on Glucose Oxidase-Attached Polyion Complex Membrane Containing Microperoxidase and Ferrocene

Abstract

A glucose oxidase-attached polyion complex membrane containing microperoxidase and ferrocene was formed on a carbon electrode. The enzyme electrode gave a reductive current response (100% response time, within 20 s), and steady-state response was proportional to the glucose concentration up to 0.2 mM. Lower detection limit was 1 μM. The current response decreased gradually, owing to the loss of the microperoxidase activity. The enzyme electrode was used for the detection of glucose concentration in beverages. The results obtained agreed well with those obtained by Boehringer F-kit method. The electrode would be useful as a glucose biosensor for real samples.