High-throughput analysis of glycoprotein-derived glycans by using automated microchip electrophoresis system
2019 Volume 63 Issue 2 Pages 47-54
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2019 Volume 63 Issue 2 Pages 47-54
High-throughput quantitative and qualitative analytical procedures are required for glycoscientific research (e.g. metabolism of glycans, glyco-biomarker discovery, and quality control of glycoprotein pharmaceuticals). Among some analytical techniques, microchip electrophoresis is one of the candidate technique for high-throughput quantitative and qualitative glycoanalysis. We describe here a strategy for high-throughput analytical procedures for the relative quantitation of N-linked glycans by using and automated microchip electrophoresis system “MultiNA”, which is specialized for DNA/RNA analysis. We designed suitable labeling of glycans for fluorescence detection in “MultiNA” and optimized separation condition for labeled glycans without any hardware modification. 8-Aminopyrene-1,3,6-trisulfonate (APTS)-labeled N-glycans released from some glycoproteins were successfully separated based on the difference in branching type and terminal modifications within 100s. Furthermore, we also designed microchip lectin- affinity electrophoresis for glyco-profiling. Thus, automated microchip electrophoresis provides high-throughput quantitative and qualitative glycoanalytical procedures and “MultiNA” will provide a new dimension of glycoscience in the field of biology.
