2021 Volume 1 Pages 47-53
High-precision analytical methods to detect fumonisin B1 (FB1) in a limited range of biological samples have recently been reported. Here an analytical method for FB1 for use in a wider range of tissue and body fluid samples, such as the plasma, kidney, liver, urine, and bile was developed. Pigs were used in this investigation and administered with FB1 via osmotic pumps. Samples were extracted with a formic acid/acetonitrile/water mixture, purified on a phospholipid removal solid-phase extraction column, and analyzed by ultra-high performance liquid chromatography-electrospray ionization/tandem mass spectrometry (UHPLC-ESI/MS/MS). The mean recovery of FB1 in the plasma, kidney, liver, and urine ranged from 80%–115%, and the corresponding relative standard deviation (RSD) was below 10%. The matrix effects resulted in ionization suppression in the bile samples, but these effects were stable and constant, and could be accounted for; the mean recovery and RSD for the bile samples was 10% and <10%, respectively. Using this analytical method, FB1 was precisely detected in pigs and its levels decreased in the order of urine>bile>kidney=liver>plasma, which implied fast excretion. The detection and quantitation limits (1 and 3 ng g−1, respectively) alongside the other results indicated that this method was suitable for the analysis of FB1 in various types of biological samples.