Abstract
For the clinical applications of serum protein bound iodine, the method for its determation has to be more simplified and the data should be highly reliable. Along this line, the author contrived a new method applicated the distillation method.
The gaseous iodine produced by reduction was gathered directly into the solution (pH 8.3) of arsenious acid with NaHCO3. The celium solution was added to this solution which was acidified by H2SO4 for producing Ce-As system, thus the serum protein-bound iodine would be estimated by its discoloration.
At first, iodine in the blood specimen was estimated from the standard curve previously prepared, secondary, the blank values were determined as PBI values, hereafter the serum protein-bound iodine estimation was made. Results of the recovery test for this new method distributed within the ranges from 88 to 107 per cent (avarage 95 per cent). The parallel tests showed their differences from 0.2 to 0.77 per 100ml. According to these results we may conclude the date obtained by this method has high accuracy.
The normal level obtained by this method was 4.5-8.0γ per 100ml. (avarage 6.2γ per 100ml.).
The datas in patients of various diseases were obtained as follows :
Pulmonary tuberculosis : 5.5 to 13.0γ per 100ml.
hyperthyroidism and thyrotoxicosis : 8.2 to 19.3γ per 100ml.
tumor of the pituitary gland : 7.0 to 9.5γ per 100ml.
diabetes mellitus : 3. 7 to 9. 6γ per 100ml.
Struma Simplex : 5.2 to 7.3γ per 100ml.
obesity : 2.5 to 6.8γ per 100ml.
hypothyroidism (with incomplete clinical symptoms) : 1.9 to 3.5γ per 100ml.
myxoedema : 1.8 to 2.8γ per 100ml.
Simmonds' Disease : 3.6 to 4.7γ per 100ml.
Nephrosis : 3.5 to 5.6γ per 100ml. Conclusively, the author introduced a new simplified method of serum protein-bound iodine determination and reviewed the datas estimated by this method.
