Morphological and Functional Studies on the Secretory Mechanism of the Thyroid Stimulating Hormone
1961 Volume 36 Issue 12 Pages 1966-1985,1939
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1961 Volume 36 Issue 12 Pages 1966-1985,1939
On the subject of the secretory mechanism of the thyroid stimulating hormone in the anterior pituitary, there still remains a number of unsolved problems.
To clarify one of the aspects, the author has attempted an investigation of TSH content in the pituitary gland which was assayed by the modified Greenspan method and histochemical studies of the pituitary under the various experimental conditions in female rats.
The results are summarized as follows :
A) Radiothyroidectomized female rats.
1) The anterior pituitary gland showed remarkable hypertrophy after the radiothyroidectomy and TSH content of the pituitary of the radiothyroidectomized rat was as shown in Table 1.
2) On the histochemical observation, the acidophils were distinctly decreased in number for 7 days after the radiothyroidectomy and these were quite difficult to distinguish from the chromophobes after 30 days, whereas the basophils were increased in size and number after the radiothyroidectomy.
Sixty days after the radiothyroidectomy, the thyroidectomy cells appeared, containing coarse, dense granules, (T granules) which give an intense reaction with the periodic acid-Schiff reagent for carbohydrate, and at the same time there appeared a few chromophobes with the protoplasm which has an affinity for staining.
