Abstract
The effects of some hormones upon NEFA release from liver slice, diaphragm and epididymal adipose tissue were investigated. Each tissue of the Hereditary Obese-hyperglycemic Mice (C 57/6J-ob, OH) and New Zealand Obese Mice (NZO) were incubated with hormones in the medium of Krebs-Ringer Bicarbonate buffer containing 3% bovine albumin and 5% Ediol (Emultion of 50% coconut oil).
The results obtained are as follow :
1) Effects of epinephrine and norepinephrine on NEFA release from liver slice and diaphragm were similar to those of their control mice.
2) Epididymal adipose tissue from OH mice exhibited an impairment in its ability to release NEFA when incubated with epinephrine, norepinephrine and ACTH. The inhibitory action of insulin and glucose on NEFA release was also less marked in epididymal adipose tissue from OH mice than in their control mice. However, this abnormality was not found in tissue from NZO mice.
Decrease sensitivity to those hormones on NEFA release from OH mice in vitro may be one of the metabolic abnormalities in this type of hyperglycemic obesity.
