Abstract
Recently the following decapeptide sequence for porcine luteinizing releasing hormone (LRH) was proposed by Schally's group : (pyro) Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2. According to this report, many investigators have succeeded in synthetizing LRH and this synthetic LRH is now available for clinical study. Then, synthetic LRH of Daiichi Pharmaceutical Company, Tokyo, Japan was tested in the human being. Two hundred micrograms of synthetic LRH was injected into the following 8 women and serum LH responce was tested by double antibody radioimmunoassay (RIA) : 2 volunteers of follicular phase, 2 volunteers of luteal phase, 2 anovulatory females of amenorrhea with estrogenic activity and 2 anovulatory females of amenorrhea without estrogenic activity. Single iv injection of 200 micrograms of synthetic LRH induced a significant increase in LH release into the blood within 30 minutes in 7 of 8 subjects. Only one exception was a case of hypopituitarism and no responce of LH release was to be found after injection. This study demonstrate that synthetic LRH will be utilized effectively for the study on the function of hypothalamo-pituitary gonadal axis. Moreover, induction of ovulation by synthetic LRH and inhibition of ovulation by antibody of LRH is promising in the human being.
