1974 Volume 50 Issue 4 Pages 766-773,748
The influence starvation and alloxan treatment on the butyrate metabolism, and the relationship between the butyrate metabolism and glycolysis were changed by the starvation and alloxan treatment in sheep liver slices have already been reported. Further study was designed to elucidate the relationship between the butyrate metabolism and TCA cycle, and how this relationship was influenced by the starvation and alloxan treatment in sheep liver slices.
The results obtained are summeri'ed as follows.
1) In liver slices of normally fed sheep, the 14C transfer from 14C-butyrate into glucose and NEFA was decreased and the formation of 14C-cholesterol, -triglyceride and -phospholipid from 14C-butyrate was increased by the addition of citrate or succinate. And the formation of 14CO2 and 14C-phospholipid from 14C-citrate was increased and the 14C transfer from 14C-citrate into glucose, ketone bodies, cholesterol and triglyceride was decreased by the butyrate addition, and the formation of 14CO2 and 14C-ketone bodies form 14C-succinate was increased and the 14C transfer from 14C-succinate into glucose, cholesterol, triglyceride and phospholipid was decreased by the butyrate addition.
2) In liver slices of starved sheep, the 14C tranfer from 14C-butyrate into CO2, cholesterol, triglyceride and phospholipid was increased and the formation of 14C-glucose and -NEFA was decreased by the addition of citrate or succinate. And the formation of 14CO2, 14C-glucose and -phospholipid from 14C-citrate was increased and the 14C transfer from 14C-citrate into ketone bodies, cholesterol and triglyceride was decreased by the butyrate addition, and the formation of 14CO2, 14C-glucose and -ketone bodies form 14C-succinate was increased and the 14C transfer from 14C-succinate into cholesterol, triglyceride and phospho-lipid was decreased by the butyrate addition.
3) In liver slices of alloxan diabetic sheep, there were no effects of the citrate addition on the 14C transfer from 14C-butyrate into CO2, ketone bodies and lipid fractions and also there were no effects of the butyrate addition on the 14C transfer from 14C-citrate into all these fractions. But the formation of 14C-glucose and -NEFA from 14C-bytyrate was decreased and the 14C transfer from 14C-bytyrate into cholesterol, triglyceride and phospholipid was increased by the succinate addition. And the formation of 14CO2 and -ketone bodies from 14C-succinate was increased and the 14C transfer from 14C-succinate into glucose, cholesterol, triglyceride and phospholipid was decreased by the butyrate addition.
4) In liver slices of normally fed, starved and alloxan diabetic sheep, there were no effects of the addition of citrate or succinate on the formation of 14C-ketone bodies from 14C-butyrate, and also there were no effects of the butyrate addition on the 14C-NEFA formation from 14C-citrate or -succinate.
As mentioned above, it may be deduced that there are some relationships between the butyrate metabolism and TCA cycle and these relationships between the butyrate metabolism and TCA cycle were changed by the starvtion or alloxan treatment in sheep liver slices.
