An Immunochemical Assay of Pregnanediol 3-glucuronide in Urine and its Variation in the Peri-ovulatory Period

Abstract

A rapid, simple and sensitive immunochemical assay of pregnanediol 3-glucuronide (Pd-3G) in urine was established. The standard error range of the recovery test was within 0.8 μg/ml, and the coefficient variation of the within run and day to day precision tests were within 4%. Cross reactivity of various steroids exhibited less than 4%. A significant rise of Pd-3G (mg) /creatinine (g) coincided with the LH surge and continued during the luteal phase in the ovulatory menstrual cycle. In an anovulatory cycle, Pd-3G was found to be under 4 mg/g. This Pd-3G direct assay method could be widely used for assessing ovulation and luteal function.