Abstract
Distribution survey methods of macro-organisms using environmental DNA（eDNA）have been rapidly developing in recent years. Environmental DNA analysis can be divided into two major methods, species-specific detection and eDNA metabarcoding. Here we outline these techniques, and we report the preliminary results of an eDNA-based biomass survey of Japanese jack mackere（l Trachurus japonicus）. The results showed significantly positive associations between eDNA concentrations from water samples and echo intensity detected by an echo sounder, suggesting that the spatial variation of the eDNA concentration can reflect the local biomass of fish even in marine environments. More detailed distribut ion mapping of coastal fish can be achieved by environmental DNA analysis, and it will contribute in various fields, such as fisheries science, ichthyology, and ecology.
