Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 0007-5124
Histochemical Demonstration of Proteins, Lipids and Polysaccharides in Japanese Monkey Oocytes
Sueo NIIMURANobuyoshi FUJISAWANorimitsu L. SATO
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JOURNAL FREE ACCESS

1985 Volume 34 Issue 1 Pages 73-76

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Abstract
A histochemical study of proteins, lipids and polysaccharides was carried out in the oocytes of Japanese monkey (Macaca fuscata) during development of follicles. There were a small number of protein granules reactive to acrolein-Schiff in the cytoplasm of oocytes from primordial, secondary and vesicular follicles, while there were no lipid droplets, granules of neutral polysaccharides or acid polysaccharides in the cytoplasm. Proteins reactive to acrolein-Schiff, neutral polysaccharides reactive to periodic acid-Schif f and acid polysaccharides stainable with alcian blue were observed in the zona pellucida of the oocytes of secondary and vesicular follicles. The zona pellucida contained sudanophilic lipids composed of neutral fats and lipoids, besides the proteins and polysaccharides.
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© Japanese Association for Laboratory Animal Science
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