Method of Separating Mouse Platelets by Discontinuous Sucrose-gradient Centrifugation

Abstract

A discontinuous sucrose gradient was employed in the separation of mouse blood platelets using a modified Booyse method. The platelets of male CD-1 mice aged 8 to 12 weeks were divided into five distinct populations (A, B, C, D & E) . Distribution of light to heavy platelets patterns in 10 normal CD-1 mice was demonstrable at; A (S.G. 1.188), as 14.8±5.6%; B (S.G. 1.199), 44.0±4.6%; C (S.G. 1.207), 24.1±3.4 %; D (S.G. 1.214), 13.0±3.6%; and E (S.G. 1.221), 4.0±1.5%.