Genetical and Histological Studies on a Mouse Displaying Microphthalmia and Cataract

Abstract

A male mouse displaying bilateral microphthalmia and cataract was found among the offspring of pregnant Slc: ICR mouse treated intraperitoneally with 10 mg/kg methylnitrosourea on gestational day 4. This mutant has been maintained by brother-sister mating. By the mating test with normal Slc: ICR mice, this character was revealed to be inherited by an autosomal single recessive gene. Linkage test with the brown locus showed that this mutant gene is linked with the B gene and mapped on chromosome 4. The histological study of the eyes of adult mutant mice revealed various abnormalities all over the eyes, especially in the lens and neural retina. Embryologically, the mutant mice showed persistent connection between the lens vesicle and the surface ectoderm by a cellular stalk, and also the formation of retinal infolding, in the early stages of eye development. Both were considered to be responsible for the consequent abnormal development and degradation of the lens. These results suggest that the mutant mouse we found may be an allele of the dysgenetic lens (dyl) reported by Sanyal and Hawkins [2] .