Studies on the Development of an ELISA Kit for Microbiological Monitoring

2. Improvement of the Prototype ELISA Kit with Special References to Mouse Hepatitis Virus Antigen

Abstract

Improvement of the mouse hepatitis virus (MHV) antigen in a prototype ELISA kit was performed. Equivalent divalent antigens of MHV Nu-67 and S strains with a protein concentration of 10μg/ml showed the best sensitivity and specificity for the detection of MHV and sialodacryoadenitis/Parker's rat coronavirus antibodies in mice and rats, respectively. An increase in the reliability of macroscopic evaluation of both antibody tests is expected by using the newly manufacturedkit with the improved antigen.