A New Method of Long-term Bile Collection from Unrestrained Dogs

Abstract

A method of long-term bile collection from unrestrained dogs was developed. A steel needle was passed subcutaneously from the animal's right side into the abdomen. A catheter, threaded through this needle, was inserted from the side and brought out below the abdomen. The catheter was introduced into the common bile duct and a vest was then fitted on the dog. The external catheter tip was placed in a collection bottle in the pocket of the vest. This method allows the animal to move freely in its cage. The bile of an unrestrained dog weighing 10kg was collected for 3 weeks at an output rate of 4.2-4. 6ml per hour.