1997 Volume 13 Pages 47-52
We developed a new in vitro assay for chemosensitivity test using collagen gel droplet embedded culture and image analysis (CD-DST method) . In this in vitro assay, we successfully minimized the cancer cell number required for culture apporoximately 3-10×103 cells for each 30μl collagen gel droplet, obtained the sufficient growth of cancer using serum free medium while suppressing the growth of fibroblast cells. It became possible to measure the volume of cancer cells by neglecting the contaminating fibroblast cells by the Neutral Red-stain and an image analysis in assessment. The evaluability of in vitro assay for 282 surgical specimens of several primary cancers was 85%, and the accuracy for in vitro-in vivo correlation in 11 cases was 91%. Tumor cells isolated from human tumor xenografts implanted into nude mice and cultured by CD-DST were exposed to drugs under conditions that can reproduce the plasma AUC in vitro. The in vitro sensitivity to a drug was compared with the in vitro response of the same tumor treated with the MTD of the drug. When the criterion of sensitivity was taken as 50% or less of the growth inhibition, the correlation between the in vitro and in vivo growth inhibition of MMC, CDDP and ADM tested was relatively high (86% of the true positive rate, 82% of the ture negative rate and 83% of the correlation rate) .
Bulletin of the Experimental Animals
Proceedings of The Japanese Association of Animal Models for Human Diseases
