Abstract
Cellular polyamines acid-extracted
from newly available 31 strains of the class Halobacteria,
one strain of the class Methanobacteria, one strain of the
class Methanocococi, five strains of the class
Methanomicrobia, one strain of the class
Thermoplasmata and three strains of the class
Thermococci of the phylum Euryarchaeota, and two
strains of the class Thermoprotei of the phylum
Crenarchaeota, and one strain of the class
Nitrososphaeria and one strain of the class
Conexivisphaeria of the phylum Thaumarchaeota, were
additionally analyzed by HPLC and HPGC-MS. The
anaerobic sulfur-respirating extremely- halophilic
archaea Halanaeroarchaeum and Natrarchaeobaculum
were poor in agmatine, spermidine, and spermine.
Extremely-thermophilic methanogenic
Methanofervidicoccus abyssi contained spermidine,
spermine, and a quaternary branched penta-amine,
N4
-bis(aminopropyl)spermidine. In thaumarchaeotes,
mesophilic ammonia-oxidizing Nitrosopumilus zosterae
contained putrescine and homospermidine, whereas
thermoacidophilic, sulfur- and iron-reducing
Conexivisphaera calida contained putrescine, spermidine
and spermine. Two alkanolpolyamines,
N-(2-hydroxyethyl)-1,3-diaminopropane
(N-(3-aminopropyl)aminoethanol) and
N-(3-hydroxypropyl)-1,3-diaminopropane
(N-(3-aminopropyl)aminopropanol) were detected in
thermoacidophilic Sulfuracidifex metallicus belonging to
the class Thermoprotei. New polyamine data were
combined into our final archaeal polyamine distribution
catalogue for a chemotaxonomy in the domain Archaea.
