Abstract
Recently, in vitro synthetic biology has been
actively engaged in the reconstitution of artificial
systems implemented with life-like characteristics to
understand the principle and origins of life. One of the
most important characteristics of life is
self-reproducibility, which is originated from the
self-regeneration ability of the gene replication and
expression system (i.e., central dogma). In this review,
we summarize recent progress in in vitro reconstitution
of biological functions that constitute central dogma,
DNA replication, transcription, and translation.
