2000 Volume 56 Issue 9 Pages 456-461
Enzymatic hydrolysis of cotton fiber was studied in detail to find out a good way to monitor the weight loss of fiber precisely. In this enzymatic treatment the linear relationship between the weight loss and the glucose formation was confirmed. Stoichiometric analysis of the hydrolysis products revealed that both glucose and oligosaccharides are formed from cellobiose in a constant ratio to give the linearity. This may be attributed to the cellobiose hydrase and polymerase activities involved in the cellulase used (originated from Filamentous fungi). Based on this finding, a new monitoring system using the chemi-luminescence of luminol was examined for assessing the glucose formation, which was related with the total weight loss of cotton fiber. This method is superior to other methods in terms of shortness of measuring time and applicable for monitoring the weight loss of dyed fabrics.
