Abstract
Organized molecular films adsorbed several bio-materials have been prepared by Langmuir-Blodgett method using an organo-modified montmorillonite. In this method, organo-montmorillonite films play a template role of the lysozyme thin layer. Chemisorption of lysozyme to the anionic montmorillonite surface has supported by infrared spectra of multilayers. The bands of N-H bending and C=O stretching vibrations of lysozyme were clearly identified in the spectra of multilayers coated with chemisorbed lysozyme. The morphology of these film surfaces were observed by atomic force microscopy at mesoscopic scales. In the case of organo-modified montmorillonite adsorbed protease, it is also indicated that activity of chemisorbed protease was maintained up to 160 ℃ of annealing temperature. The thickness of the enzyme layer was also maintained. This conservation of steric structure of enzyme is ascribable to the formation of aggregate of adsorbed enzyme on the montmorillonite surface and the construction of sandwiched structure of enzyme between organo-montmorillonite.
