Abstract
Cells of Trichoderma viride, a cellulolytic fungus, were immobilized in chitosan gel beads and employed for producing cellulase by a repeating batch procedure. The activity of the immobilized cells increased with extending culture period, and reached a maximum, 37 μg of reducing sugars per mL of culture filtrate, in 6 days. This value was about 1/4 of that for free cells. The enzyme activity of chitosan gel-immobilized cells in the repeated, batch culture retained about 50% of the initial activity after ten times of batch culture.
