Abstract
To elucidate the action mechanism of bioactive sulfated polysaccharides which exhibited anticoagulant and antiviral activities, interactions of an anticoagulant polysaccharide heparin (Hep) with polylysine (PL) which can be regarded as a model compound for an anticoagulant protein antithrombin-III, respectively, were examined by NMR spectroscopy. It was found that a gellike complex was formed by the ionic interaction of Hep with PL. Since in 1H NMR spectra of the mixture of Hep and PL, absorptions due to the lysine side chain appeared as two species which were due to excess free polylysine and gellike complex, the proportion of the complex was determined. The proportion of the gellike complex was affected by _??_w of heparin and polylysine, molar ratio, pH, concentration, temperature, and time. Maximal amount of the gellike complex was attained with Hep having 3×103 Da and PL having 10×103 Da.
