Abstract
A 3×3 factorial experiment was conducted using three levels of supplemental L-ascorbyl-2-phosphate-Mg (APM) 0, 10 and 1, 000mg/100g of diet and three levels of n-3 highly unsaturated fatty acids (n-3 HUFA) 0, 1 and 2% of diet on Japanese flounder Paralichthys olivaceus juveniles (initial body weight 0.35g). Average body weight was about 6-7g over a 50-day period of feeding in the APM-supplemented groups with 1 and 2% of n-3 HUFA, these groups showed higher weight gain and feed conversion efficiency than in the APM-unsupplemented groups. Analysis of variance on weight gain, feed conversion efficiency and concentration of ascorbic acid in the liver revealed that there were significant (p<0.05) interactions between APM and n-3 HUFA levels. The fatty acid composition of total lipid in whole-body, neutral and polar fractions in the liver almost mirrored that of thedietary lipids. Inspite of the APM level, there was no marked difference among the groups of diets with thesame level of n-3 HUFA. Regardless of n-3 HUFA level in the diets, the total lipid concentration in whole-body and liver generally increased due to the accumulation of triglyceride when the APM level increased.
