Introduction of pBIN19 Plasmid Gene into the Marine Origin Pseudomonas SC9 Using Electroporation

Abstract

Introduction of new functions to a marine bacterium was aimed at by using a plasmid vector. The plasmid pBIN 19, derived from the broad host range plasmid RK 2, was introduced into marine isolated Pseudomonas strain SC 9 by electroporation. Despite the low transformation efficiency of the bacterial conjugation method reported previously, a transformation efficiency of 1.81×10⁴transformants/μg of pBIN 19 was obtained for the strain SC 9 by electroporation. The introduced pBIN 19was not detected in plasmid form in transformed cell. Southern blot hybrids appeared in genomic DNA under 50 kb using crossed field gel electrophoresis, which indicated introduction and recombination of pBIN 19 into genomic DNA of Pseudomonas SC 9.