Abstract
To obtain restriction fragment length polymorphism (RFLP) and variable number of tandem repeat (VNTR) markers from the rainbow trout genome, we constructed a genomic cosmid library from the RTG-2 cell line. Cosmid DNAs were prepared from 25 clones and examined to detect DNA polymorphisms. Three conventional RFLPs and 5 VNTR markers were identified. One of the VNTR markers (RVF9301) consisted of repeat units of a minisatellite tandem repeat of 52 bp inlength. In a primary study for cytogenetic mapping, we succeeded in localizing these markers on rainbow trout chromo-somes by fluorescence in situ hybridization. Our results suggest these 8 clones will serve as useful genetic markers for linkage analysis of genetic traits in fisheries science.
