A New Purification Method of G-actin Extracted from the Acetone-Dried Myofibril-Powder Using Ammonium Sulfate Fractionation. A Comparison with the Conventional G-actin and High-Salt Sensitivity

Abstract

A new preparative method of purified G-actin from myofibril of carp dorsal muscle was developed. Myofibril suspended in 0.1M KCl was treated with acetone and converted into dried myofibril-powder. Polymerizable G-actin was extracted from the powder with ATP containing solution. G-actin was purified by collecting the pellet at 40% saturated ammonium sulfate. The G-actin purified by the above procedures was indistinguishable from the conventional G-actin in its purity, yield, and abilities to polymerize and to interact with myosin. G-actin thus prepared was proved to be resistant to high-salt (such as 40% saturated ammonium sulfate, 4.5M NaCl or 3.5M KCl) treatment.