cDNA Cloning of Polypeptide Chain Elongation Factor 1α from Medaka Oryzias latipes

Masato Kinoshita; Takahiro Nakata; Kousuke Adachi; Taijiro Yabe; Yoshihiro Yokoyama; Haruhiko Toyohara; Takashi Hirata; Eiji Takayama; Satoshi Mikawa; Noriyuki Kioka; Morihiko Sakaguchi

doi:10.2331/fishsci.65.133

cDNA Cloning of Polypeptide Chain Elongation Factor 1α from Medaka Oryzias latipes

Masato Kinoshita, Takahiro Nakata, Kousuke Adachi, Taijiro Yabe, Yoshihiro Yokoyama, Haruhiko Toyohara, Takashi Hirata, Eiji Takayama, Satoshi Mikawa, Noriyuki Kioka, Morihiko Sakaguchi

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Masato Kinoshita
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Takahiro Nakata
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Department of Anatomy, Faculty of Medicine, National Defense Medical College
Kousuke Adachi
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Taijiro Yabe
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Yoshihiro Yokoyama
Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University
Haruhiko Toyohara
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Takashi Hirata
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Eiji Takayama
Faculty of Medicine, National Defense Medical College
Satoshi Mikawa
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
Department of Animal Breeding and Genetics, National Institute of Animal Industry
Noriyuki Kioka
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
Morihiko Sakaguchi
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

Keywords: polypeptide elongation factor lα, EF1α, cDNA, medaka

JOURNAL FREE ACCESS

1999 Volume 65 Issue 1 Pages 133-137

DOI https://doi.org/10.2331/fishsci.65.133

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Abstract

Polypeptide chain elongation factor 1α (EF1α) was cloned from a liver cDNA library of medaka Orizias latipes. The analysis of the clone containing 1.6kbp DNA revealed that the entire open reading frame of EFla was 1383 bp long. Deduced amino acid sequence of medaka EFlα showed high similarity (more than 87% identity) with those of other vertebrates. Medaka EFlα had one amino acid deletion at the position of 447 amino acid of other EF1αs and unique 17 replacement against those of other vertebrates. Northern hybridization analysis revealed that this EF1α was expressed in all tissues tested.

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