Fisheries science
Print ISSN : 0919-9268
Isolation and Inheritance of Microsatellite Markers in the Common Carp Cyprinus carpio
Ratu Siti AliahMotohiro TakagiShi DongChin Tick TeohNobuhiko Taniguchi
Author information
JOURNAL FREE ACCESS

1999 Volume 65 Issue 2 Pages 235-239

Details
Abstract
Three microsatellite loci, Cca-17*, Cca-21*, and Cca-30*, were isolated from a partial genome library of common carp Cyprinus carpio. Primers for PCR amplification of template DNA were designed based on the unique sequences flanking each motif. The total number of alleles detected from nishikigoi and wild type common carp population were five, six and nine at loci Cca-17*, Cca-21*, and Cca-30*, respectively. Inheritance was shown to be Mendelian by analysis of genotype ratio in F1 offspring of six families of nishikigoi. Allele *69 at locus Cca-21* and allele *266 at locus Cca-30* were detected only in wild type common carp with frequencies of 0.304 and 0.458, respectively, while allele *288 was observed only in the nishikigoi with the frequencies ranged from 0.600-0.610.
Content from these authors
© The Japanese Society of Fisheries Science
Previous article Next article
feedback
Top