Taxonomical and Serological Studies on the Causative Bacteria of the Disease of Sea Urchin Strongylocentrotus intermedius Occurring at Low Water Temperatures

Kenjiro Takeuchi; Kenichi Tajima; Mohammed Mahbub Igbal; Tomoo Sawabe; Yoshio Ezura

doi:10.2331/fishsci.65.264

Taxonomical and Serological Studies on the Causative Bacteria of the Disease of Sea Urchin Strongylocentrotus intermedius Occurring at Low Water Temperatures

Kenjiro Takeuchi, Kenichi Tajima, Mohammed Mahbub Igbal, Tomoo Sawabe, Yoshio Ezura

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Keywords: sea urchin, bacterial disease, identification, Vibrio spp, low water temperatures

JOURNAL FREE ACCESS

1999 Volume 65 Issue 2 Pages 264-268

DOI https://doi.org/10.2331/fishsci.65.264

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Published: 1999 Received: September 24, 1998 Available on J-STAGE: June 30, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 30, 2008 Reason for correction: - Correction: CITATION Details: Wrong : K. Kanai: “Togenukesho” of sea urchins. Proceedings of the Symposium on “Diseases in fish and shellfish culture in Kyushu and Okinawa” Japanese Society of Fish Pathology, 1993, p. 7.
1) M. Hamaguchi, I. Kawahara, and H. Usuki: Mass mortality of Pseudocentrotus depressus caused by a bacterial infection in summer. Suisanzoshoku, 41 189-193 (1993) (in Japanese).
2) K. Tajima, T. Hirano, M. Shimizu, and Y. Ezura: Isolation and pathogenicity of the causative organisms of spotting disease of sea urchin Strongylocentrotus intermedius. Fisheries Sci., 63, 249-252 (1997).
3) K. Tajima, K. Takeuchi, M. M. Iqbal, K. Nakano, M. Shimizu, and Y. Ezura: Studies on a bacterial disease of sea urchin Strongylocentrotus intermedius occurring at low water temperatures. Fisheries Sci., 64, 918-920 (1998).
4) Editorial committee of a handbook of Microbiology: A handbook of microbiology, Gihodo, Tokyo, 1957, p. 621 (in Japanese).
5) R. Sakazaki: Bacterial culture media. Vol. 1, Kindaishuppan, Tokyo, 1978, pp. 404 (in Japanese).
6) T. Kimura: A method for rapid detection of alginic acid digesting bacteria. Bull. Fac. Fish. Hokkaido Univ., 12, 41-48 (1961) (in Japanese).
8) J. Marmur: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol., 3, 208-218 (1961).
9) M. Kumagai, M. Fujimoto, and A. Kuninaka: Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate. Nucleic Acids Res., 19, 65-68 (1988).
10) T. Ezaki, Y. Hashimoto, and E. Yabuuchi: Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacterial., 39, 224-229 (1989).
11) T. Sawabe, Y. Oda, Y. Shiomi, and Y. Ezura: Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. Microb Ecol., 30, 193-202 (1995).
12) H. Urakawa, K. Kita-Tsukamoto, and K. Ohwada: 16S rDNA genotyping using PCR/ RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae. FEMS Microbiol. Lett., 152, 125-132 (1997).
13) K. Kita-Tsukamoto, H. Oyaizu, K. Nanba, and U. Simidu: Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol., 43, 8-19 (1993).

Right : K. Kanai: “Togenukesho” of sea urchins. Proceedings of the Symposium on “Diseases in fish and shellfish culture in Kyushu and Okinawa” Japanese Society of Fish Pathology, 1993, p. 7.
1) M. Hamaguchi, I. Kawahara, and H. Usuki: Mass mortality of Pseudocentrotus depressus caused by a bacterial infection in summer. Suisanzoshoku, 41 189-193 (1993) (in Japanese).
2) K. Tajima, T. Hirano, M. Shimizu, and Y. Ezura: Isolation and pathogenicity of the causative organisms of spotting disease of sea urchin Strongylocentrotus intermedius. Fisheries Sci., 63, 249-252 (1997).
3) K. Tajima, K. Takeuchi, M. M. Iqbal, K. Nakano, M. Shimizu, and Y. Ezura: Studies on a bacterial disease of sea urchin Strongylocentrotus intermedius occurring at low water temperatures. Fisheries Sci., 64, 918-920 (1998).
4) Editorial committee of a handbook of Microbiology: A handbook of microbiology, Gihodo, Tokyo, 1957, p. 621 (in Japanese).
5) R. Sakazaki: Bacterial culture media. Vol. 1, Kindaishuppan, Tokyo, 1978, pp. 404 (in Japanese).
6) T. Kimura: A method for rapid detection of alginic acid digesting bacteria. Bull. Fac. Fish. Hokkaido Univ., 12, 41-48 (1961) (in Japanese).
7) F. Yanagisawa: Chitinase, _??_Choenbiburio_??_ (K. Fujino-H. Fukumi), Isseido, Tokyo, 1964, pp. 215-221 (in Japanese).
8) J. Marmur: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol., 3, 208-218 (1961).
9) M. Kumagai, M. Fujimoto, and A. Kuninaka: Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate. Nucleic Acids Res., 19, 65-68 (1988).
10) T. Ezaki, Y. Hashimoto, and E. Yabuuchi: Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacterial., 39, 224-229 (1989).
11) T. Sawabe, Y. Oda, Y. Shiomi, and Y. Ezura: Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. Microb Ecol., 30, 193-202 (1995).
12) H. Urakawa, K. Kita-Tsukamoto, and K. Ohwada: 16S rDNA genotyping using PCR/ RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae. FEMS Microbiol. Lett., 152, 125-132 (1997).
13) K. Kita-Tsukamoto, H. Oyaizu, K. Nanba, and U. Simidu: Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol., 43, 8-19 (1993).

Date of correction: June 30, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Identification of the causative bacteria Vibrio spp., which were isolated from diseased sea urchin Strongylocentrotus intermedius and their rearing cages at low seawater temperatures at Date, Shiriuchi and Shikabe Fisheries Breeding Centers in Hokkaido was undertaken based on their biochemical properties, DNA-DNA homology and serological analysis. All fifteen strains, five from each Breeding Center were almost identical in biochemical properties except indole production. However, the strains isolated at Date differed from the strains at Shiriuchi and Shikabe in DNA-DNA homology and analysis of thermostable and thermolabile antigenic compositions. These results indicated that the strains from Shiriuchi and Shikabe belonged to the same species but they were different from those of Date. Moreover, the causative Vibrio spp. differed in biochemical, genotypical and serological properties from any strains of Vibrio spp. isolated from the intestine of healthy sea urchin and any of type strains of the genus Vibrio.

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