Isolation of Greater Amberjack Microsatellite DNA and Their Application as Genetic Marker to Species of Genus Seriola from Japan

Abstract

Six primer sets were designed from the greater amberjack, Seriola dumerili, and tested to greater amberjack and three other species of genus Seriola. The primer sets tested have only scorable product in samples from the same genus and may not be available to amplify DNA from the other genera. Four of those loci were polymorphic for greater amberjack, and three loci were polymorphic for other species of genus Seriola, with number of alleles ranging from 2 to 16, and observed heterozygosities between 0.350 to 0.800. Species S. lalandi has the highest variability, while the lowest belongs to species S. rivoliana. Genetic distance, calculated over those loci for all species samples, shows a clear clarification of species according to the taxonomic arrangement. These loci show potential as genetic markers for assessment of genetic variability and population genetic study of species of genus Seriola.