Abstract
The effect of bisphenol A and nonylphenol on carp Cyprinus carpio phagocytic cells was analyzed in vitro. Head kidney leukocytes of carp were exposed to 10 nM of bisphenol A or nonylphenol, separately. Subsequently, the production of superoxide anions was measured via the reduction of nitroblue tetrazolium (NBT) in vitro. Macrophages incubated with 0.1 nM up to 10 nM of bisphenol showed an increase in the production of superoxide anions in comparison with the control macrophages, which were not treated with bisphenol A. Nonylphenol also induced the production of superoxide anions in phagocytic cells prepared from carp kidney. Phagocytic cells treated with both bisphenol A and nonylphenol decreased phagocytic activity. However, the production of nitric oxide in carp macrophages did not change when treated only with either bisphenol A or nonylphenol. These results indicate that bisphenol A and nonylphenol affected the function of phagocytic cells in vitro.
