Abstract
Genome analysis was conducted to detect the virulence genes in Pasteurella piscicida. To date we have analyzed 366 clones from plasmid genomic library and 507 clones from cosmid genomic library, from which we determined a total of 932, 722 by nucleotide sequences. As a result of comparison of these clones to the GenBank database, five clones from plasmid library and 11 clones from cosmid library have homology with deduced bacterial virulence genes. In the plasmid library, these are: DsbA, LspA2, nucleotide sugar epimerase, OpsX and PIdA . In the cosmid library, these are: delta endotoxi, hemolysin, heme receptor, hyahronidase, immature fimbrillin, lipidA dissacharide synthase, lipidA 4'-kinase, neurotoxin, PIdA, TonB and Wzz. We consider that these results are useful information to identify virulence genes in P. piscicida, that will now allow further analysis of this bacterium's pathogenicity.
