2002 Volume 68 Issue sup2 Pages 1210-1212
A cDNA encoding a rainbow trout homologue of mammalian heart-type FABP was first isolated. Its sequence information was obtained from nucleotides (nt)-53 to +597, excluding the poly (A) tail, and an open reading frame of 399 nt was shown coding for 133 amino acids. A polyadenylation consensus signal was found 24 nt upstream from poly (A) tail. The coding sequence was interrupted by three introns of 2, 218, 891 (887) and 137 nt. Mammalian heart-type FABP has been known to be distributed in various tissues, except for liver, while reverse transcription-polymerase chain reaction showed high expression of heart-type FABP in liver, heart, muscle, intestine and brain of rainbow trout. The expression of heart-type FABP in fish liver seemed to be associated with lipid accumulation.
