Perception of light intensity by Haliotis discus discus based on locomotor activity patterns

Abstract

An apparatus to measure the locomotor activity of aquatic benthic organisms at variable low light levels was developed and the diurnal behavioral pattern of the abalone Haliotis discus discus was measured at various low light intensities. During the experiment, abalone were exposed to 12 h light-dark cycles of complete darkness, 0 μmol/m²/s throughout the 12 h dark cycle and, during periods I (days 1-8) and III (days 19-26), the 12 h light cycles were set at 10 μmol/m²/s. During period II (days 10-17), abalone were exposed to a light level during the 12 h light cycles of 1×10^-5, 1×10^-6, 1×10^-7 or 1×10^-8 μmol/m²/s and the changes in locomotor activity assessed. At daytime levels of 1×10^-5 μmol/m²/s, typical behavioral patterns were observed of high locomotory activity during the night-time cycle. However, at lower light intensities, the distinction between day and night activity patterns became less clear and, at intensities lower than 1×10^-7 μmol/m²/s, the difference between activity during the light and dark cycles became negligible. Based on this, we conclude that the threshold of light level perception in relation to locomotor activity is approximately 1×10^-7 μmol/m²/s. The significance of these results in relation to the entrainment of behavior in abalone is discussed.